Laboratory of Electron Microscopy is equipped with high resolution scanning electron microscope with field emission, with ion beam column, micromanipulator, SEM-EDX detector, STEM detector and EsB® detector attached.
Microscope allows for precise „in situ” preparation of biological material directly in its chamber, both in room temperature and in cryo conditions.
We provide the preparation of samples for observations in SEM and STEM,
elemental composition and distribution analysis, preparation of ultrathin sections for TEM, observations using serial block-face SEM for three-dimensional reconstruction.
Observations of biological specimens using field emission SEM
– max. resolution ~0,8 nm
Observations of biological specimens
using transmission electron microscopy STEM
– magnifications up to 2 million times
– up to 12 specimens for series
Observations of native biological material
using highly sensitive, energy-selective backscattered electron detector ESB® at normal and cryo conditions
– nano-scaled clear compositional contrast
– work below 1,5 kV allowing for selecting the desired energy of backscattered electrons
Analysis of biological specimens
by serial imaging of the surfaces revealed by gallium ion milling
– collection of serial images desired for three-dimensional reconstruction of subcellular features
Analysis of elemental composition and distribution
within the biological specimens using energy-dispersive
TEM lamella preparations using focused ion beam and micromanipulations
– „in situ” preparation of ultrathin sections for TEM observations with thickness below 30 nm
CROSS-BEAM SCANNING ELECTRON
microscope with ion beam Auriga 60, Zeiss
Ultramicrotome EM UC7, Leica
Cryo chamber FC7, Leica
Automatic contrasting device EM AC20, Leica
High vacuum sputter EM ACE600, Leica
Tissue processor EM TP, Leica
dr hab. Krzysztof Marycz – Head of Laboratory
dr Jakub Grzesiak – Research Engineer
- Grzesiak J, et al. (2017) Ultrastructural changes during osteogenic differentiation in mesenchymal stromal cells cultured in alginate hydrogel. Cell & Bioscience 7(1):2.
- Marycz K, et al. (2016) Polyurethane/Polylactide-Blend Films Doped with Zinc Ions for the Growth and Expansion of Human Olfactory Ensheathing Cells (OECs) and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) for Regenerative Medicine Applications. Polymers 04/2016; 8(5). DOI:10.3390/polym8050175.
- Marędziak M, et al. (2016) Static magnetic field enhances the viability and proliferation rate of adipose tissue-derived mesenchymal stem cells potentially through activation of the phosphoinositide 3-kinase/Akt (PI3K/Akt) pathway. Electromagnetic Biology and Medicine 07/2016; DOI:10.3109/15368378.2016.1149860.
- Marycz K, et al. (2016) Sphingosine-1-Phosphate Enhance Osteogenic Activity of Multipotent Stromal Cells Cultured in Biodegradable 3D Alginate Hydrogels. Journal of Biomaterials and Tissue Engineering 03/2016; 6(2). DOI:10.1166/jbt.2016.1423.
- Marycz K, et al. (2016) Macro autophagy and selective mitophagy ameliorate chondrogenic differentiation potential in adipose stem cells (ASC) of equine metabolic syndrome (EMS) – new findings in the field of progenitor cells differentiation. Oxidative Medicine and Cellular Longevity in press.
- Marycz K, et al. (2016) Polyurethane/polylactide-blend films doped with zinc ions for the growth and expansion of human olfactory ensheathing cells (OECs) and adipose-derived mesenchymal stromal stem cells (ASCs) for regenerative medicine applications. Polymers 8: 175.
- Grzesiak J, et al. (2015) Polyurethane/polylactide-based biomaterials combined with rat olfactory bulb-derived glial cells and adipose-derived mesenchymal stromal cells for neural regenerative medicine applications. Materials Science and Engineering C 147: 163-170 .
- Grzesiak J, et al. (2015) Characterization of olfactory ensheathing glial cells cultured on polyurethane/polylactide electrospun nonwovens. International Journal of Polymer Science 2015: 908328.
- Marycz K, et al. (2014) Influence of modified alginate hydrogels on mesenchymal stem cells and olfactory bulb-derived glial cells cultures. Bio-Medical Materials and Engineering; 24: 1625-1637.
- Marycz K, et al. (2014) Polyurethane/polylactide-based electrospun nonwovens as carriers for human adipose derived stromal stem cells and chondrogenic progenitor cells. Polymer-Plastics Technology and Engineering 06/2016; DOI:10.1080/03602559.2016.1163586
Autor: PORT - Polski Ośrodek Rozwoju Technologii, Opublikowano: 09.05.2016