Mass Spectrometry Laboratory (MS)

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Mass Spectrometry Laboratory (MS)

The Mass Spectrometry Laboratory provides a state-of-the-art infrastructure and expertise for proteomic, metabolomic and imaging mass spectrometry analyses for research and commercial partners. We specialize in method development for our partners and invite you to joint participation in research projects in cooperation with scientific institutions and industrial partners.

We offer identification and quantitative analysis of organic compounds in the areas of:

  • PROTEOMICS: „bottom up” peptide analysis for protein identification, posttranslational modification (PTMS) analysis: phosphorylation and glycosylation, protein quantitation (SRM/MRM), de novo protein identification, „top down” analysis of intact proteins
  • METABOLOMICS: profiling and identification of metabolites in clinical samples (serum, urine) and tissue material (MALDI imaging), analysis of low molecular weight substances
  • LIPIDOMICS: separation and identification of lipids and related compounds, profiling of cellular and membrane lipids of plant, animal and human origin
  • PHARMACOKINETICS:  DMPK analysis of drug metabolites, method development for identification of known drugs and novel compounds in food and cosmetics
  • MALDI imaging: identification and analysis of distribution of biological compounds in tissues

 

Our laboratory has implemented a quality standard PN-EN ISO/IEC 17025:2005.

ms

  • HIGH-RESOLUTION HYBRID FT MASS SPECTROMETER LTQ Orbitrap Elite ETD (Thermo Fisher Scientific)
    – Mass analyzers: LTQ (linear ion trap) and Orbitrap
    – Interchangeable ion sources: ESI (HESI), APCI and nano
    – CID, PQD, HCD and ETD fragmentation
    – FAIMS ion mobility module
    – Chromatograph Easy nano-LC 1000 and Ultimate 3000 (Dionex)

 

  • MAXIS IMPACT MASS SPECTROMETER (BRUKER)
    – mass analyzers: Quadrupole and TOF
    – CID fragmentation
    – Interchangeable ion sources: ESI, APCI, Captive Spray
    – Chromatographs Ultimate 3000 RSLC (Dionex) and Easy -nLC (Bruker)
    – Software: Data Analysis 4.1, MetaboliteTools 3.0
  • ULTRAFLEXTREME MASS SPECTROMETER (BRUKER)
    – Biotyper for identification of microorganisms (bacteria, yeast)
    – ImagePrep for MALDI imaging of tissues
    – CID and LIFT fragmentation
    – Proteineer IIfc sample preparation robot
    – FlexAnalysis, ClinProTool
  • SOFTWARE FOR DATA ANALYSIS:
    – Mascot Server (Matrix Science)
    – Proteome Discoverer 3.0 (Thermo)
    – ProteinScape 3.1 (Bruker)

Elżbieta Piątkowska PhD – Laboratory Manager

Paweł Pasikowski PhD – Senior Testing engineer

Anna Czajkowska PhD – Testing engineer

Katarzyna Kapczyńska PhD – Testing engineer

Piotr Biniarz – Process engineer

 

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Posted by PORT - Polski Ośrodek Rozwoju Technologii, Posted on 08.10.2015
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