MICROBIOLOGY

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MICROBIOLOGY

Microbiology is our passion. We offer a wide spectrum of services starting from food and microbiology, through anti-microorganism activity analysis, including anti-biofilm, medicines and medical products and ending with production of bacterial cellulose. We have large experience not only in work using normative methods, but also in creating non-standard testing systems reflecting customer requirements. We are a part of the research unit of the Polish Technology Development Centre, which has at its disposal the most modern research equipment, we are able to take up any microbiological challenge, which you put in from of us.
WHAT DISTINGUISHES US
At the PORT Microbiology Laboratory we will perform not only all the services determined in the standards and guidelines, but also we will provide you with reliable and repeatable results in testing, for which the protocols are yet to be developed and which require also application of advanced chemical methods, imaging or performed in real time.

 

  • The assessment of microorganism susceptibility in the planktonic form and biofilm to standard use and experimental antimicrobial means (antibiotics, antiseptics, peptides, plant derived active substances and synthetic ones) – with normative and experimental methods
  • Microbiological purity assessment of supplied samples (medicines, medical products, pharmaceuticals, cosmetics, food, environmental samples and others)
  • Surface antimicrobial properties assessment
  • Surface colonization assessment
  • Preparation of samples for analysis and microorganism identification with the MALDI-TOF-MS method
  • Testing of microbiological sterility with the direct culture method acc. to FP
  • Assaying the number of microorganisms (bacteria and mycete) in samples
  • Assessment of microorganism drug susceptibility
  • Assessment of interactions between microorganisms
  • Sample lyophilising
  • Assessment of anti-biofilm effectiveness of active dressing, antiseptics and other products using the Biofilm Oriented Antiseptic Test (B.O.A.T.) method and Antibiofilm Dressing Activity Measurement (A.D.A.M.)
  • Assessment of anti-microorganism anti-biofilm activeness of volatile compounds
  • Assessment of anti-microorganism activeness of products in specific reaction environments (exudate, blood, urine, etc.)
  • Works with obligatory anaerobe microorganisms – selected assaying upon consulting the customer
  • Isolation and identification of pathogens and non-pathogenic microorganisms from food, environmental and other samples
  • Production of agar media with nonstandard composition according to the Customer demand
  • Assessment of material ability to sequestration of pathogens
  • Assaying of properties selected by the customer and features of the microorganisms cultured in flow conditions.
  • Production and purification of bacterial cellulose
  • Coating of medical samples with bacterial cellulose
  • Creating of experimental systems reflecting customer demand and analysis of the received results
  • Preparation of samples for analysis and microorganism identification with the MALDI-TOF-MS, PB-MIKRO-01 ed. method 2 dated 24.07.2018 and
  • Testing of microbiological sterility with the direct culture method acc. to FP, ed. X, tom 1, 2014, point 2.6.27 or 2.6.1 I-MIKRO-01 ed. 2 dated 24.07.2018.
  • Laminar flow cabinets SCANLAF MARS
  • PHYSIOLOGICAL CELL CULTURE WORK STATIONS INVIVO2 500 BAKER RUSKIN
  • Incubation shakers HT Multitron PRO
  • Centrifuge Eppendorf 5424R – 5820R
  • ThermoMixers C Eppendorf
  • Lyophilizer Labconco FreeZone
  • Ultra-low temperature freezer (-80°C) Binder
  • Incubators- including CO2 Binder incubators
  • pH-meters, analysis weighing scales
  • Spiral plater – Eddy Jet IUL
  • MEDIAJET system

Adam Junka PhD – Laboratory Manager
Bartłomiej Dudek PhD – Testing Engineer

Joanna Czajkowska, MSc Eng. (postgraduate student) – Scientific-Technical Issues Specialist

The PORT Microbiology Laboratory has existed since September 2017. Within the first 6 months we participated in preparation, and now we perform the following projects in the fields of basic science and applications :

  1. OPUS NCN 2017/27/B/NZ6/02103 : Analysis of increased effectiveness mechanisms of anti-microbial substances towards biofilm at presence of centrifugal magnetic field
  2. NCBIR POIR.04.01.04-00-0012/17. Green technology of production of succinic acid from renewable and waste materials
  3. Implementation doctorate executed by the Polish Centre of Technology Development and West Pomeranian University of Technology: Fibroblast response to presence of biofilms formed by pathogens isolated from persistent vein ulceration

The employees of the PORT Microbiology Laboratory are also co-authors of the submitted patent application NDW/522-55-1/18 : The way of production of modified bacterial cellulose in the culturing medium supplemented with plant oil.

In 2018 the Microbiology Laboratory participated in the program: “Good internships” enabling taking part in practices for 6 students of the Wrocław University.

Science is our passion. Below we present selected publishings of the team members from the last 5 years:

  • Junka, R. Rakoczy, P. Szymczyk, M. Bartoszewicz, P. P. Sedghizadeh, K. Fijałkowski.: Application of rotating magnetic fields increase the activity of antimicrobials against wound biofilm pathogens Sci.Rep. 2018 Vol.8; art.167 [12 s.] IF: 4.122 Point. MNiSW: 40,000
  • Anna Żywicka, Adam Junka, Patrycja Szymczyk, Grzegorz Chodaczek, Jakub Grzesiak, Parish Paymon Sedghizadeh, Karol Fijałkowski.: Bacterial cellulose yield increased over 500% by supplementation of medium with vegetable oil. Carbohydr.Polym. 2018199; s.294-30 IF: 5.158 Point. MNiSW: 40,000
  • Adam Junka, Karol Fijałkowski, Adam Ząbek, Katarzyna Mikołajewicz, Grzegorz Chodaczek, Patrycja Szymczyk, Danuta Smutnicka, Anna Żywicka, Parish Paymon Sedghizadeh, Mariusz Dziadas, Piotr Młynarz, Marzenna Bartoszewicz.: Correlation between type of alkali rinsing, cytotoxicity of bio-nanocellulose and presence of metabolites within cellulose membranes Carbohydr.Polym. 2017 157; s.371-379IF: 5.158 Point. MNiSW/KBN: 40,000
  • Parish P. Sedghizadeh, Shuting Sun, Adam Junka, Eric Richard, Keivan Sadreraf, Susan Mahabady, Neema Bakhshalian, Natalia Tjokro, Marzenna Bartoszewicz, Monika Oleksy, Patrycja Szymczyk, Mark W. Lundy, Jeffrey D. Neighbors, R. Graham G. Russell, Charles E. McKenna, Frank H. Ebetino.: Design, synthesis, and antimicrobial evaluation of a novel bone-targeting bisphosphonate-ciprofloxacin conjugate for the treatment of osteomyelitis biofilms J.Med.Chem. 2017 Vol.60 no.6; s.2326-2343IF: 6.253 Point. MNiSW/KBN: 45,000
  • Adam Junka, Patrycja Szymczyk, Grzegorz Ziółkowski, Ewa Karuga-Kuźniewska, Danuta Smutnicka, Iwona Bil-Lula, Marzenna Bartoszewicz, Susan Mahabady, Parish Paymon Sedghizadeh.: Bad to the bone: on in vitro and ex vivo microbial biofilm ability to directly destroy colonized bone surfaces without participation of host immunity or osteoclastogenesis PLoS One 2017 Vol.12 no.1; art.e0169565 [20 s.] IF: 2.766 Point. MNiSW/KBN: 35,000
  • Futoma-Koloch B., Bugla-Płoskońska G., Dudek B., Dorotkiewicz-Jach A., Drulis-Kawa Z., Gamian A. Outer membrane proteins of Salmonella as potential markers of resistance to serum, antibiotics and biocides”, Current Medicinal Chemistry (2018) 25: 1. https://doi.org/10.2174/0929867325666181031130851 (IF 3,5; point. MNiSW 35)
  • Pawlak A.; Rybka J.; Dudek B.; Krzyżewska E.; Rybka W.; Kędziora A.; Klausa E.; Bugla-Płoskońska G. Salmonella O48 serum resistance is connected with the elongation of the lpopolysaccharide O-antigen containing sialic acid. Int. J. Mol. Sci. 2017, 18, 2022. (IF 3,2; point. MNiSW 30)
  • Futoma-Kołoch, B., Dudek, B., Kapczyńska, K., Krzyżewska, E., Wańczyk, M., Korzekwa, K., Rybka, J., Klausa, E., Bugla-Płoskońska, G. Relationship of Triamine-Biocide tolerance of Salmonella enterica serovar Senftenberg to antimicrobial susceptibility, serum resistance and outer membrane proteins. Int. J. Mol. Sci. 2017; 18, 1459. (IF 3,2; point. MNiSW 30)
  • Dudek B., Krzyżewska E., Kapczyńska K., Rybka J., Pawlak A., Korzekwa K., Klausa, E., Bugla-Płoskońska, G. Proteomic analysis of outer membrane proteins from Salmonella Enteritidis strains with different sensitivity to human serum. PLoS ONE 2016; 11(10): e0164069. https://doi.org/10.1371/journal.pone.0164069. (IF 2,8; point. MNiSW 40)
  • Skorek K., Raczkowska A., Dudek B., Miętka K., Guz-Regner K., Pawlak A., Klausa E., Bugla-Płoskońska G., Brzostek K.: Regulatory protein OmpR influences the serum resistance of Yersinia enterocolitica O:9 by modifying the structure of the outer membrane. PLoS ONE 2013; 8(11):e79525. Doi:10.1371/journal.pone.0079525. (IF 3,2; point. MNiSW 40)
  • Mieczkowski A, Psurski M, Bagiński M, Bieszczad B, Mroczkowska M, Wilczek M, Czajkowska J, Trzybiński D, Woźniak K, Wietrzyk J. Novel (S)-1,3,4,12a-tetrahydropyrazino[2,1-c][1,4]benzodiazepine-6,12(2H,11H)-dione derivatives: Selective inhibition of MV-4-11 biphenotypic B myelomonocytic leukemia cells’ growth is accompanied by reactive oxygen species overproduction and apoptosis. Bioorg Med Chem Lett. 2018 Feb 15;28(4):618-625. doi: 10.1016/j.bmcl.2018.01.034. Epub 2018 Jan 31. IF=2.442, point. MNiSW=30
  • Joanna Czajkowska, Adam Junka, Agata Markowska-Szczupak Application of the metabolomics as the future tool in quick diagnostic of persistent wounds. Wound Treatment 2018;15(1):1–44. Wound Treatment 2018
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Posted by Polski Ośrodek Rozwoju Technologii, Posted on 28.11.2018
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