Protein Engineering Laboratory
The Protein Engineering and Particle Reacting Laboratory is comprehensively equipped with the most modern equipment for testing properties and functions of proteins with biochemical and biophysical methods. We offer the possibility of recombinant proteins production and purification from different expression platforms. Moreover, the laboratory is prepared for the analysis of reactions occurring between proteins, proteins and DNA, ligands and peptides. We have at our disposal specialist equipment enabling measurement of bioactivity and kinetic parameters in a wide range from pM to mM.
We perform testing within scientific projects and commercial orders.
Contact: inquiries@port.org.pl
Production and purification of recombinant proteins from different expression systems
Qualitative and quantitative protein bioanalysis:
- Identification (MS, MS/MS)
- Purity (PAGE, WB, SEC, UV-Vis, HPLC/FPLC)
- Activity (SPR, BLI, MST, ELISA, AlphaScreen, AlphaLISA, UV-NIR, FRET, TR-FRET, TRF, FP)
- Aggregation/oligomerization (DLS, SEC)
- Stability (DSC, CD)
- Structural analysis (CD)
Protein designing/modification in order to obtain desired properties/activities
Testing of molecular reacting
size of analysed reagents from ions to viruses
Kinetics and reaction mechanism
Kd, kon, koff
Kd, DH, DS, stoichiometry
Enzyme kinetics parametrization
kcat, Vmax, Km, IC50, Ki
Bioactivity testing
SPR, BLI, MST
ELISA, AlphaScreen, AlphaLISA
UV-NIR, FRET, TR-FRET, TRF, FP
Selection, characterisation and optimization of potential bio therapeutics
Apparatus for measuring interaction between molecules:
Biacore T200
Apparatus using surface plasmon resonance (SPR)
Octet RED384
Device using layered interferometry (BLI)
Monolith NT.115Pico
Device using microscale thermophoresis (MST)
Dionex Ultimate 3000
Dionex Ultimate 3000
AKTA Explorer
AKTA Explorer
Tecan FreedomEVO 150
Multifunctional workstation for cleaning biopreparations with a plate reader Infinite M1000 Pro
Tecan FreedomEVO 200
Automatic station for testing ligand receptor interactions with a multifunctional reader Infinite M200 Pro
MicroCal VP-Capillary DSC
Automated differential scanning calorimeter
MicroCal ITC200
Isothermal titration calorimeter
Zetasizer DLS/SLS Nano ZS
Zetasizer DLS/SLS Nano ZS
Jasco J-815
Apparatus for measuring the rotation of CD light
Plate readers:
PE Enspire with modul Corning Epic, PE Victor X4, Spectra MaxPlus 384
Spectrofluorimetr Sinco FS-2
Spectrophotometer Evolution 201
Autor: abachmatiuk, Opublikowano: 10.02.2016
