Protein Engineering Laboratory

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Protein Engineering Laboratory

The Protein Engineering and Particle Reacting Laboratory is comprehensively equipped with the most modern equipment for testing properties and functions of proteins with biochemical and biophysical methods. We offer the possibility of recombinant proteins production and purification from different expression platforms. Moreover, the laboratory is prepared for the analysis of reactions occurring between proteins, proteins and DNA, ligands and peptides. We have at our disposal specialist equipment enabling measurement of bioactivity and kinetic parameters in a wide range from pM to mM.

We perform testing within scientific projects and commercial orders.

Contact: inquiries@port.org.pl

Production and purification of recombinant proteins from different expression systems

Qualitative and quantitative protein bioanalysis:

  • Identification (MS, MS/MS)
  • Purity (PAGE, WB, SEC, UV-Vis, HPLC/FPLC)
  • Activity (SPR, BLI, MST, ELISA, AlphaScreen, AlphaLISA, UV-NIR, FRET, TR-FRET, TRF, FP)
  • Aggregation/oligomerization (DLS, SEC)
  • Stability (DSC, CD)
  • Structural analysis (CD)

Protein designing/modification in order to obtain desired properties/activities

Testing of molecular reacting

size of analysed reagents from ions to viruses

Kinetics and reaction mechanism

Kd, kon, koff

Kd, DH, DS, stoichiometry

Enzyme kinetics parametrization

kcat, Vmax, Km, IC50, Ki

Bioactivity testing

SPR, BLI, MST

ELISA, AlphaScreen, AlphaLISA

UV-NIR, FRET, TR-FRET, TRF, FP

Selection, characterisation and optimization of potential bio therapeutics

Apparatus for measuring interaction between molecules:

Biacore T200

Apparatus using surface plasmon resonance (SPR)

 

Octet RED384

Device using layered interferometry (BLI)

 

 Monolith NT.115Pico

Device using microscale thermophoresis (MST)

 

Dionex Ultimate 3000

Dionex Ultimate 3000

 

AKTA Explorer

AKTA Explorer

 

Tecan FreedomEVO 150

Multifunctional workstation for cleaning biopreparations with a plate reader Infinite M1000 Pro

Tecan FreedomEVO 200

Automatic station for testing ligand receptor interactions with a multifunctional reader Infinite M200 Pro

 

MicroCal VP-Capillary DSC

Automated differential scanning calorimeter

 

MicroCal ITC200

Isothermal titration calorimeter

 

Zetasizer DLS/SLS Nano ZS

Zetasizer DLS/SLS Nano ZS

 

Jasco J-815

Apparatus for measuring the rotation of CD light

Plate readers:

PE Enspire with modul Corning Epic, PE Victor X4, Spectra MaxPlus 384

Spectrofluorimetr Sinco FS-2

Spectrophotometer Evolution 201

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Posted by abachmatiuk, Posted on 10.02.2016
SINGLE LABORATORIUM
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